3ILB
Crystal structure of mouse Bcl-xl mutant (R139A) at pH 6.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-08 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.497, 34.721, 95.552 |
| Unit cell angles | 90.00, 89.62, 90.00 |
Refinement procedure
| Resolution | 35.450 - 2.380 |
| R-factor | 0.2394 |
| Rwork | 0.237 |
| R-free | 0.29340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pq0 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.768 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.470 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.140 | 0.140 |
| Number of reflections | 12366 | |
| <I/σ(I)> | 25 | 4.5 |
| Completeness [%] | 91.1 | 91.4 |
| Redundancy | 4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 1.2M Ammonium sulphate, 0.3M Lithium sulphate, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
