Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2007-09-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.28244 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 142.841, 147.197, 121.172 |
Unit cell angles | 90.00, 109.52, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
Rwork | 0.240 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dkf |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
Rmerge | 0.049 | 0.033 | 0.346 |
Number of reflections | 81525 | ||
<I/σ(I)> | 16.3 | ||
Completeness [%] | 99.9 | 99.2 | 100 |
Redundancy | 5.4 | 5 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 50mM sodium citrate, 0.2M ammonium sulfate, 0.6M lithium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |