3HIH
Structure of human Plk1-PBD with glycerol and sulfate in the phophopeptide binding site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-08-09 |
Detector | MAR scanner 300 mm plate |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.300, 102.300, 68.500 |
Unit cell angles | 90.00, 93.20, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.700 |
R-factor | 0.188 |
Rwork | 0.187 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.551 |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.055 | 0.259 |
Number of reflections | 49465 | |
<I/σ(I)> | 23.1 | 2.9 |
Completeness [%] | 98.4 | 85.8 |
Redundancy | 4.8 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.2 M lithium sulfate monohydrate, 0.1 M bis-tris, 25% w/v PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |