3HCU
Crystal structure of TRAF6 in complex with Ubc13 in the C2 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97912 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 130.493, 41.387, 123.745 |
Unit cell angles | 90.00, 116.20, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.600 |
R-factor | 0.2556 |
Rwork | 0.256 |
R-free | 0.33290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hct |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
Rmerge | 0.046 | 0.032 | 0.394 |
Number of reflections | 18484 | ||
<I/σ(I)> | 55.287 | ||
Completeness [%] | 98.8 | 98.6 | 94.1 |
Redundancy | 6.8 | 6.8 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 16-20% PEG 4000, 0.2M Magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |