3GV5
Human DNA polymerase iota in complex with T template DNA and incoming ddADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Collection date | 2008-03-07 |
Wavelength(s) | 0.98322 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 140.160, 71.750, 127.420 |
Unit cell angles | 90.00, 112.53, 90.00 |
Refinement procedure
Resolution | 51.570 - 2.000 |
R-factor | 0.206 |
Rwork | 0.205 |
R-free | 0.25300 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.958 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 51.570 | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 5.290 | 1.950 |
Rmerge | 0.072 | 0.024 | 0.617 |
Number of reflections | 84496 | ||
<I/σ(I)> | 24.607 | ||
Completeness [%] | 99.0 | 98.2 | 96.3 |
Redundancy | 3.6 | 3.7 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 7.5 | 298 | 15% PEG 3350, 0.15 M CaCl2, 5% glycerol, 0.005M DTT, pH 7.5, hanging drop, temperature 298K |