3G6V
DNA synthesis across an abasic lesion by human DNA polymerase-iota
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-05 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 98.150, 98.150, 203.315 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.220 - 2.200 |
R-factor | 0.21399 |
Rwork | 0.211 |
R-free | 0.25146 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fll |
RMSD bond length | 0.024 |
RMSD bond angle | 2.266 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.058 | 0.428 |
Number of reflections | 29220 | |
<I/σ(I)> | 37.6 | 37.6 |
Completeness [%] | 97.0 | 100 |
Redundancy | 10.1 | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Crystals grew over a period of one day, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |