3FE2
Human DEAD-BOX RNA helicase DDX5 (P68), conserved domain I in complex with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-30 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9184 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 84.570, 106.870, 117.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.910 - 2.600 |
R-factor | 0.2084 |
Rwork | 0.205 |
R-free | 0.27283 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2i4i |
RMSD bond length | 0.012 |
RMSD bond angle | 1.354 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.670 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.142 | 0.742 |
Number of reflections | 16601 | |
<I/σ(I)> | 11 | 2.44 |
Completeness [%] | 99.4 | 99.8 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 277 | 17.5% PEG3350, 0.25M Lithium Sulfate, 0.1M Bis-Tris, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K |