3DBI
CRYSTAL STRUCTURE OF SUGAR-BINDING TRANSCRIPTIONAL REGULATOR (LACI FAMILY) FROM ESCHERICHIA COLI COMPLEXED WITH PHOSPHATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2008-03-30 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 82.468, 82.468, 341.448 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.450 |
R-factor | 0.23211 |
Rwork | 0.231 |
R-free | 0.27042 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.193 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.096 | 0.870 |
Number of reflections | 44638 | |
<I/σ(I)> | 5.4 | 1.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13 | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 20% PEG3350 PH 7.0, 100MM DI-POTASSIU HYDROGEN PHOSPHATE, 10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K, pH 5.00 |