3CZA

Crystal Structure of E18D DJ-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9
Spacegroup name	P 31 2 1
Unit cell lengths	74.826, 74.826, 74.777
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.000 - 1.200
R-factor	0.1305
Rwork	0.131
R-free	0.16370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p5f
RMSD bond length	0.014
RMSD bond angle	0.030
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.000	1.240
High resolution limit [Å]	1.200	1.200
Rmerge	0.069	0.695
Number of reflections	75880
<I/σ(I)>	35.5	2.9
Completeness [%]	99.9	100
Redundancy	11.1	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	1.5 M sodium citrate, 50 mM HEPES, 10 mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K