3CQS
A 3'-OH, 2',5'-phosphodiester substitution in the hairpin ribozyme active site reveals similarities with protein ribonucleases
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-04-07 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 93.160, 93.160, 129.050 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.920 - 2.800 |
R-factor | 0.22 |
Rwork | 0.217 |
R-free | 0.25400 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2p7f |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.200 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 8458 | |
<I/σ(I)> | 15.5 | 1.4 |
Redundancy | 5.09 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20.5% (w/v) PEG 2000 MME, 0.10 M Na cacodylate buffer, 0.25 M Li2SO4, 2.5 mM Co(NH3)6Cl3, 2 mM spermidine-HCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 2000 MME | ||
2 | 1 | 1 | Li2SO4 | ||
3 | 1 | 1 | spermidine | ||
4 | 1 | 1 | sodium cacodylate | ||
5 | 1 | 1 | Co(NH3)6Cl3 | ||
6 | 1 | 2 | PEG 2K MME | ||
7 | 1 | 2 | Li2SO4 | ||
8 | 1 | 2 | HEPES | ||
9 | 1 | 2 | spermidine |