2Y70
CRYSTALLOGRAPHIC STRUCTURE OF GM23, MUTANT G89D, AN EXAMPLE OF CATALYTIC MIGRATION FROM TIM TO THIAMIN PHOSPHATE SYNTHASE.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-01-27 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 32 |
Unit cell lengths | 109.444, 109.444, 95.240 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.448 - 2.300 |
R-factor | 0.2014 |
Rwork | 0.199 |
R-free | 0.23040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y6z |
RMSD bond length | 0.008 |
RMSD bond angle | 1.162 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.060 | 0.410 |
Number of reflections | 55952 | |
<I/σ(I)> | 12.9 | 2.4 |
Completeness [%] | 98.7 | 99.9 |
Redundancy | 2.1 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | PROTEIN WAS CRYSTALLIZED FROM 2M LI2SO4, 100 MM MES, PH 6.5 |