2XRZ

X-ray structure of archaeal class II CPD photolyase from Methanosarcina mazei in complex with intact CPD-lesion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-08
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	71.700, 116.200, 168.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.480 - 2.200
R-factor	0.17093
Rwork	0.170
R-free	0.20819
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	STRUCTURAL DATA OF NATIVE M. MAZEI PHOTOLYASE.
RMSD bond length	0.012
RMSD bond angle	1.363
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.480	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.080	0.690
Number of reflections	72130
<I/σ(I)>	17.5	2.6
Completeness [%]	99.9	100
Redundancy	4.9	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6		0.2 M AMMONIUM SULPHATE, 0.1 M SODIUM ACETATE PH 4.6, 10% (W/V) PEG 4000, 10% (V/V) GLYCEROL