2X6M
Structure of a single domain camelid antibody fragment in complex with a C-terminal peptide of alpha-synuclein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 173 |
Detector technology | IMAGE PLATE |
Detector | MAR555 FLAT PANEL |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 60.170, 63.120, 62.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.560 - 1.620 |
R-factor | 0.1596 |
Rwork | 0.156 |
R-free | 0.20258 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hcv |
RMSD bond length | 0.018 |
RMSD bond angle | 1.693 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.710 |
High resolution limit [Å] | 1.620 | 1.620 |
Rmerge | 0.120 | 0.540 |
Number of reflections | 15482 | |
<I/σ(I)> | 23.3 | 4.3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 14.3 | 14.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 25% PEG6000, 100 MM HEPES PH 7.5, 100 MM LICL |