2WXQ
The crystal structure of the murine class IA PI 3-kinase p110delta in complex with AS15.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-21 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 142.130, 63.860, 116.630 |
Unit cell angles | 90.00, 102.62, 90.00 |
Refinement procedure
Resolution | 65.940 - 2.700 |
R-factor | 0.22673 |
Rwork | 0.225 |
R-free | 0.28623 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rd0 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.424 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0046) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.340 | 2.720 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.100 | 0.540 |
Number of reflections | 28229 | |
<I/σ(I)> | 11.35 | 1.69 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 3.47 | 3.56 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.8 | 20% (V/V) GLYCEROL, 10% (W/V) PEG 4K, 30 MM NANO3, 30 MM NA2HPO4, 30 MM (NH4)2SO4, 100 MM IMIDAZOLE PH 6.8 |