2VZE
Crystal structure of human acyl-CoA synthetase medium-chain family member 2A (L64P mutation) in complex with AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Collection date | 2008-03-18 |
Detector | RIGAKU AREA DETECTOR |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 97.730, 97.730, 384.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.160 - 2.450 |
R-factor | 0.196 |
Rwork | 0.193 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3b7w |
RMSD bond length | 0.013 |
RMSD bond angle | 1.452 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.990 | 2.710 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.010 | 0.490 |
Number of reflections | 73402 | |
<I/σ(I)> | 9.58 | 2 |
Completeness [%] | 98.1 | 97.1 |
Redundancy | 3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2M AMMONIUM ACETATE, 0.1M TRIS PH 8.5, 25% PEG 3350 |