2VFA
Crystal structure of a chimera of Plasmodium falciparum and human hypoxanthine-guanine phosphoribosyl transferases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-17 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 161.811, 161.811, 161.811 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.233 |
| Rwork | 0.230 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hmp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.937 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.110 | 0.890 |
| Number of reflections | 17496 | |
| <I/σ(I)> | 28.5 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.8 | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1M TRIS PH 8.0, 2.0M AMMONIUM SULPHATE |
