2Q6W
The structure of HLA-DRA, DRB3*0101 (DR52a) with bound platelet integrin peptide associated with fetal and neonatal alloimmune thrombocytopenia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 98 |
Detector technology | CCD |
Collection date | 2002-01-31 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 92.169, 92.169, 248.555 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.050 - 2.250 |
R-factor | 0.214 |
Rwork | 0.210 |
R-free | 0.26543 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dlh |
RMSD bond length | 0.029 |
RMSD bond angle | 2.478 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.308 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.120 | 0.360 |
Number of reflections | 48049 | |
<I/σ(I)> | 3.04 | |
Completeness [%] | 94.0 | 55.7 |
Redundancy | 15.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 297 | 10-20% PEG 8000, 0.1M sodium acetate, 10% (v/v) glycerol, 0.01M Cd2+, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 297.0K |