2OVP
Structure of the Skp1-Fbw7 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 123 |
Detector technology | CCD |
Collection date | 2004-06-20 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9623 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 222.702, 222.702, 102.883 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.940 - 2.900 |
R-factor | 0.235 |
Rwork | 0.235 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Skp1 b-TrCP1 and Cdc4 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.093 | 0.344 |
Number of reflections | 29166 | |
<I/σ(I)> | 18.4 | 6.5 |
Completeness [%] | 99.6 | 100 |
Redundancy | 7.8 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 0.1 M HEPES-Na, 1.2 M Li2SO4, 7.5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 294K |