2OUV

crystal structure of pde10a2 mutant of D564N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-15
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	51.441, 82.205, 155.242
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.560
Rwork	0.209
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pde10a2 native
RMSD bond length	0.004
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.560
Rmerge	0.064
Number of reflections	91666
<I/σ(I)>	12.7
Completeness [%]	97.0
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1 M HEPES, pH 7.5, 0.1 M MgCl2, 100 mM BME, and 13% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K