2JFK
Structure of the MAT domain of human FAS with malonyl-CoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-19 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.917, 91.410, 261.609 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.960 - 2.400 |
R-factor | 0.224 |
Rwork | 0.222 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jfd |
RMSD bond length | 0.012 |
RMSD bond angle | 1.415 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.070 | 0.610 |
Number of reflections | 78546 | |
<I/σ(I)> | 17.3 | 2.62 |
Completeness [%] | 99.0 | 92.4 |
Redundancy | 7.22 | 6.13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 16% PEG10K 0.16M MGCL2 0.08 M TRIS PH=8.5, pH 8.50 |