2FSV
Structure of transhydrogenase (dI.D135N.NAD+)2(dIII.E155W.NADP+)1 asymmetric complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.934 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.912, 73.871, 205.172 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.370 - 2.300 |
R-factor | 0.2039 |
Rwork | 0.202 |
R-free | 0.24808 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hzz |
RMSD bond length | 0.007 |
RMSD bond angle | 1.184 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.400 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 50105 | |
<I/σ(I)> | 5.7 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.5 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 16-20% 8K-PEG, 20-150 mM (NH4)2SO4,100 mM Mes pH 6.0 and 10% glycerol in the presence of 50 mM NAD+ and 5 mM NADP+, VAPOR DIFFUSION, SITTING DROP, temperature 277K |