2FIR
Crystal structure of DFPR-VIIa/sTF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Detector technology | CCD |
Collection date | 2003-04-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.10 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.020, 80.980, 126.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.000 |
Rwork | 0.207 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.090 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 41633 | |
Completeness [%] | 84.0 | 79.9 |
Redundancy | 18.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% PEG 4000, 100 mM magnesium chloride, 200 mM sodium chloride, 10 mM calcium chloride, 20 uM zinc chloride , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |