2FD0
HIV-1 DIS kissing-loop in complex with lividomycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-10 |
Detector | MARRESEARCH |
Wavelength(s) | 0.91946 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 27.137, 115.335, 95.254 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 9.940 - 1.800 |
R-factor | 0.245 |
Rwork | 0.245 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fcy |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 26564 | |
<I/σ(I)> | 26.4 | 3.5 |
Completeness [%] | 99.3 | 98.7 |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 310 | MPD, KCl, MgCl2, Na Cacodylate pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 310K |