2D1X
The crystal structure of the cortactin-SH3 domain and AMAP1-peptide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-12 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 71.450, 132.140, 61.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.040 - 1.900 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.092 | 0.409 |
Number of reflections | 23138 | |
<I/σ(I)> | 12.8 | 2.9 |
Completeness [%] | 99.6 | 100 |
Redundancy | 5.7 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.25 | 293 | ammonium sulfate, sodium chloride, Hepes, pH 7.25, VAPOR DIFFUSION, HANGING DROP, temperature 293K |