2ALZ
Ternary Complex of hPoli with DNA and dCTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2004-10-01 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 98.114, 98.114, 202.823 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
Rwork | 0.228 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t3n |
RMSD bond length | 0.050 |
RMSD bond angle | 3.190 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.080 | 0.437 |
Number of reflections | 20339 | |
<I/σ(I)> | 15 | 6.6 |
Completeness [%] | 99.0 | 100 |
Redundancy | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 16% PEG 5K MME, 0.4 M Ammonium Sulfate, 0.1 M MES buffer, pH 6.5, hanging drop, pH 6.50 |