2A1S
Crystal structure of native PARN nuclease domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-30 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9791 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 205.540, 123.016, 82.844 |
Unit cell angles | 90.00, 112.59, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.21971 |
Rwork | 0.219 |
R-free | 0.25362 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a1r |
RMSD bond length | 0.013 |
RMSD bond angle | 1.334 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.079 | 0.628 |
Number of reflections | 58173 | |
<I/σ(I)> | 6.2 | 2 |
Completeness [%] | 99.5 | 99.6 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG3350, 0.2M ammonium tartrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |