1ZWH
Yeast Hsp82 in complex with the novel Hsp90 inhibitor Radester amine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | .99997 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 73.884, 73.884, 110.621 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.240 - 1.650 |
Rwork | 0.196 |
R-free | 0.20900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 37024 | |
<I/σ(I)> | 54.2 | 2.18 |
Completeness [%] | 98.5 | 84.9 |
Redundancy | 13.1 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5 | 291 | CaCl2, Glycerol, PEG MME 550, Na ACetate or Na Succinate, pH 5.0, Hanging Drop Vapor Diffusion, temperature 291K, Micro-batch |