1YJW
Crystal Structure Of Quinupristin Bound To The G2099A Mutant 50S Ribosomal Subunit Of Haloarcula Marismortui
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-09 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 211.690, 299.779, 573.885 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.980 - 2.900 |
R-factor | 0.171 |
Rwork | 0.171 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.000 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.132 | 0.618 |
Number of reflections | 330497 | |
<I/σ(I)> | 9.4 | 1.9 |
Completeness [%] | 92.6 | |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.8 | PEG 6000, KCL, NH4CL, MGCL2, KACETATE, PH 5.8, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 292K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 6000 | ||
10 | 1 | 2 | MgCl2 | ||
11 | 1 | 2 | Kacetate | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | NH4Cl | ||
4 | 1 | 1 | MgCl2 | ||
5 | 1 | 1 | Kacetate | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | PEG 6000 | ||
8 | 1 | 2 | KCl | ||
9 | 1 | 2 | NH4Cl |