1XL8
Crystal structure of mouse carnitine octanoyltransferase in complex with octanoylcarnitine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9790 |
Spacegroup name | H 3 |
Unit cell lengths | 163.610, 163.610, 159.480 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.090 - 2.200 |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xl7 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | COMO |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 80628 | |
Completeness [%] | 99.9 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 100mM Hepes, 62%v/vMPD, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K |