1WQ1
RAS-RASGAP COMPLEX
Experimental procedure
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-18 |
Temperature [K] | 277 |
Detector technology | AREA DETECTOR |
Collection date | 1997-03-14 |
Detector | SIEMENS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.900, 41.100, 89.000 |
Unit cell angles | 90.00, 108.40, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.500 |
R-factor | 0.233 |
Rwork | 0.233 |
R-free | 0.31900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | RAS:GPPNP (PDB ENTRY 5P21) GAP-334 (PDB ENTRY 1WER) |
RMSD bond length | 0.008 |
RMSD bond angle | 24.700 * |
Data reduction software | XDS ((W.KABSCH)) |
Data scaling software | XDS ((W. KABSCH)) |
Phasing software | X-PLOR (3.8) |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.066 * | |
Total number of observations | 35339 * | |
Number of reflections | 15811 | |
<I/σ(I)> | 10 | 1.1 |
Completeness [%] | 89.0 | 64 |
Redundancy | 2.2 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 | used to seeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | HEPES | 20 (mM) | |
3 | 1 | drop | 2 (mM) | ||
4 | 1 | drop | 20 (mM) | ||
5 | 1 | reservoir | PEG3350 | 15-20 (%) | |
6 | 1 | reservoir | HEPES | 100 (mM) | |
7 | 1 | reservoir | ammonium sulfate | 20 (mM) | |
8 | 1 | reservoir | 20 (mM) |