1W6J
Structure of human OSC in complex with Ro 48-8071
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-03-03 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 189.915, 202.424, 62.638 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 141.420 - 2.200 |
R-factor | 0.171 |
Rwork | 0.170 |
R-free | 0.19900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2sqc |
RMSD bond length | 0.009 |
RMSD bond angle | 1.180 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.100 | 0.380 |
Number of reflections | 60002 | |
<I/σ(I)> | 7.7 | 2.5 |
Completeness [%] | 98.0 | 97.5 |
Redundancy | 3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 25% (W/V) PEG3350 0.4M AMMONIUM ACETATE 0.1M TRIS PH 8.5 10% (V/V) ETHYLENE GLYCOL |