1TND
THE 2.2 ANGSTROMS CRYSTAL STRUCTURE OF TRANSDUCIN-ALPHA COMPLEXED WITH GTP GAMMA S
Experimental procedure
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.500, 108.300, 79.000 |
Unit cell angles | 90.00, 112.30, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.200 |
R-factor | 0.178 * |
Rwork | 0.190 |
R-free | 0.30400 * |
RMSD bond length | 0.011 |
RMSD bond angle | 1.800 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 12.600 * |
High resolution limit [Å] | 2.800 * |
Rmerge | 0.039 * |
Number of reflections | 53289 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 * | -12.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20-25 (mg/ml) | |
10 | 1 | reservoir | beta-mercaptoethanol | 25 (mM) | |
11 | 1 | reservoir | glycerol | 30 (%(v/v)) | |
2 | 1 | drop | sodium cacodylate | 166 (mM) | |
3 | 1 | drop | 223 (mM) | ||
4 | 1 | drop | beta-mercaptoethanol | 25 (mM) | |
5 | 1 | drop | glycerol | 20 (%(v/v)) | |
6 | 1 | drop | PEG8000 | 3.33 (%(w/v)) | |
7 | 1 | reservoir | PEG8000 | 5 (%(w/v)) | |
8 | 1 | reservoir | sodium cacodylate | 250 (mM) | |
9 | 1 | reservoir | 350 (mM) |