1REK
Crystal structure of cAMP-dependent protein kinase complexed with balanol analog 8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.600, 71.900, 98.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| Rwork | 0.218 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bx6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.190 |
| Number of reflections | 16950 | |
| Completeness [%] | 96.0 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 4 * | MPD, Bicine, Mega-8, Tris-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.8 (mg/ml) | |
| 2 | 1 | drop | BD8 | 0.3 (mM) | |
| 3 | 1 | drop | MPD | 3.3 (%) | |
| 4 | 1 | drop | bicine | 100 (mM) | pH8.0 |
| 5 | 1 | drop | Mega-8 | 0.3 (%) | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) | pH7.5 |
| 7 | 1 | reservoir | MPD | 20 (%) |
