1QUV
CRYSTAL STRUCTURE OF THE RNA DIRECTED RNA POLYMERASE OF HEPATITIS C VIRUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 100 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1998-12-08 |
| Detector | WEISSENBERG |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 63.650, 63.650, 262.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.31600 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 26.700 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | X-PLOR (98.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.500 | 2.630 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.046 | 0.064 |
| Total number of observations | 64714 * | |
| Number of reflections | 19281 | |
| <I/σ(I)> | 10.3 | 9.8 |
| Completeness [%] | 98.2 | 97.9 * |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 22.5 * | HANGING DROP, PEG 4000, SODIUM ACETATE, AMMONIUM ACETATE, TES, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 295.7K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | dithiothreitol | 5 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 21-28 (%(w/v)) | |
| 4 | 1 | reservoir | ammonium acetate | 0.2-0.35 (M) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 6 | 1 | reservoir | TES | 0.02 (M) |
