1OP8
Crystal Structure of Human Granzyme A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-06 |
Detector | MARRESEARCH |
Wavelength(s) | 1.05 |
Spacegroup name | P 1 |
Unit cell lengths | 49.480, 94.550, 94.870 |
Unit cell angles | 117.12, 100.25, 100.12 |
Refinement procedure
Resolution | 20.000 - 2.500 |
Rwork | 0.218 |
R-free | 0.28400 * |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.045 | 0.045 |
Total number of observations | 49835 * | |
Number of reflections | 44569 | |
Completeness [%] | 85.1 * | 80 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 4.7 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | ammonium sulfate | 0.1-0.3 (M) | |
2 | 1 | 1 | sodium acetate | 0.1 (M) | pH4.7 |
3 | 1 | 1 | PEG550 MME | 30-40 (%(v/v)) | |
4 | 1 | 2 | protein | 5.6-11.2 (mg/ml) |