1OOK
Crystal Structure of the Complex of Platelet Receptor GPIb-alpha and Human alpha-Thrombin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.033167 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 67.649, 67.649, 328.578 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.280 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.066 * | 0.292 * |
| Total number of observations | 608108 * | |
| Number of reflections | 35360 | |
| <I/σ(I)> | 38.6 | 5.2 |
| Completeness [%] | 99.7 | 97.5 |
| Redundancy | 17.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 4 * | PEG 6000, Ammonium Phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 2.5 (mM) | |
| 2 | 1 | drop | 50 (mM) | pH7.4 | |
| 3 | 1 | drop | protein | 25 (mg/ml) | |
| 4 | 1 | reservoir | PEG6000 | 12 (%(w/v)) | |
| 5 | 1 | reservoir | ammonium phosphate | 100 (mM) | pH7. |
