1NM9
Crystal structure of recombinant human salivary amylase mutant W58A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-09-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.946, 74.060, 134.508 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.600 * - 2.100 |
| R-factor | 0.1617 |
| Rwork | 0.160 |
| R-free | 0.19300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jxj |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.600 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.069 | 0.231 * |
| Total number of observations | 188351 * | |
| Number of reflections | 31104 | |
| <I/σ(I)> | 19.1 | |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 323 | MPD, Calcium chloride, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 323K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH9.0 |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | reservoir | MPD | 40 (%) |
