1MZJ
Crystal Structure of the Priming beta-Ketosynthase from the R1128 Polyketide Biosynthetic Pathway
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9700 |
| Spacegroup name | P 31 |
| Unit cell lengths | 96.000, 96.000, 72.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 500.000 - 2.100 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ebl |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 0.006 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.061 * | 0.587 * |
| Total number of observations | 264571 * | |
| Number of reflections | 43722 | |
| <I/σ(I)> | 8.6 | 3 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 6.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | PEG 8000, sodium cacodylate, magnesium acetate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2 (mg/ml) | |
| 2 | 1 | drop | Tris | 10 (mM) | pH7.4 |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | reservoir | PEG8000 | 10 (%(w/v)) | |
| 5 | 1 | reservoir | magnesium acetate | 200 (mM) | |
| 6 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.5 |
