1MT5
CRYSTAL STRUCTURE OF FATTY ACID AMIDE HYDROLASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-08-06 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97910 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 147.107, 272.019, 147.216 |
Unit cell angles | 90.00, 115.21, 90.00 |
Refinement procedure
Resolution | 50.000 * - 2.800 |
R-factor | 0.22062 |
Rwork | 0.218 |
R-free | 0.23700 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | FAAH structure in an alternate spacegroup. Solved by MAD/MIRAS phasing. |
RMSD bond length | 0.029 * |
RMSD bond angle | 1.041 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.800 | |
Rmerge | 0.104 * | 0.264 * |
Number of reflections | 173615 | |
<I/σ(I)> | 4.4 | |
Completeness [%] | 76.0 * | 79 * |
Redundancy | 2.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | PEG 6000, 2,4-methyl pentanediol, sodium citrate, lithium sulphate, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | reservoir | sodium citrate | 100 (mM) | pH5. |
3 | 1 | reservoir | lithium sulfate | 100 (mM) | |
4 | 1 | reservoir | PEG6000 | 7 (%(w/v)) | |
5 | 1 | reservoir | MPD | 5 (%(v/v)) | |
6 | 1 | reservoir | dithiothreitol | 0-20 (mM) |