1LPK
CRYSTAL STRUCTURE OF FXA IN COMPLEX WITH 125.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54128 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.702, 72.196, 78.612 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| Rwork | 0.192 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.490 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.065 * | 0.235 |
| Total number of observations | 37057 * | |
| Number of reflections | 15227 | |
| <I/σ(I)> | 11.8 | 3.1 |
| Completeness [%] | 89.8 | 63 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 19 * | PEG600, MES, CaCl2, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ammonium sulfate | 0.6 (mM) | |
| 2 | 1 | drop | 1 (mg/ml) | ||
| 3 | 1 | drop | benzamidine | 10 (mg/ml) | |
| 4 | 1 | drop | protein | 60 (mg/ml) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.2-1.6 (M) | |
| 6 | 1 | reservoir | pH5.0-7.0 |
