1LED
STRUCTURES OF THE LECTIN IV OF GRIFFONIA SIMPLICIFOLIA AND ITS COMPLEX WITH THE LEWIS B HUMAN BLOOD GROUP DETERMINANT AT 2.0 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 78.900, 78.900, 89.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 * - 2.000 |
| R-factor | 0.181 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.020 |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.2 * | Vandonselaar,M., (1987) J. Biol. Chem., 262, 10848. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-6 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 18-20 (%sat) | |
| 3 | 1 | drop | PIPES | 50 (mM) | pH6.2 |
| 4 | 1 | drop | sodium azide | 0.01 (%) | |
| 5 | 1 | drop | lectin | 2.2 (mol/mol) | etrasaccharide |
| 6 | 1 | reservoir | ammonium sulfate | 36-40 (%sat) | |
| 7 | 1 | reservoir | PIPES | 0.1 (M) | |
| 8 | 1 | reservoir | sodium azide | 0.02 (%) |
