1K9A
Crystal structure analysis of full-length carboxyl-terminal Src kinase at 2.5 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-09 |
| Detector | OXFORD PX210 |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 115.000, 162.600, 232.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.500 - 2.500 |
| R-factor | 0.246 |
| Rwork | 0.246 |
| R-free | 0.28800 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.427 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.000 * | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.073 | 0.398 |
| Number of reflections | 150489 | 17029 * |
| <I/σ(I)> | 6.9 | 1.7 |
| Completeness [%] | 98.2 | 98.2 |
| Redundancy | 5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 288 | ammonium sulphate, HEPES buffer, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | reservoir | HEPES-NaOH | 50 (mM) | pH7.4 |
| 3 | 1 | reservoir | ammonium sulfate | 1.90-1.95 (M) |
