1K08
Crystallographic Binding Study of 10 mM N-benzoyl-N'-beta-D-glucopyranosyl urea to glycogen phosphorylase b
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2001-04-02 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.519, 128.519, 116.497 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.750 - 2.260 |
R-factor | 0.18 * |
Rwork | 0.180 |
R-free | 0.21200 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1hlf |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.750 | 2.300 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.096 * | 0.424 * |
Total number of observations | 391254 * | |
Number of reflections | 44361 | |
<I/σ(I)> | 10.9 | 2.6 |
Completeness [%] | 95.8 | 86.5 |
Redundancy | 5.3 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 287 | Oikonomakos, N.G., (1985) Biochim. Biophys. Acta, 832, 248. * |