1JIS

CRYSTAL STRUCTURE OF TETRAGONAL LYSOZYME GROWN AT PH 4.6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	79.197, 79.197, 37.943
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.189 *
Rwork	0.190
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.005
RMSD bond angle	1.400
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.077 *	0.197 *
Total number of observations	46026 *
Number of reflections	9803	954 *
<I/σ(I)>	10.1
Completeness [%]	98.0	98.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6	293	Na-acetate buffer, pH 4.6, 10% NaCl, LIQUID DIFFUSION at 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	acetate	0.04 (M)	pH4.6