1JCM
TRPC STABILITY MUTANT CONTAINING AN ENGINEERED DISULPHIDE BRIDGE AND IN COMPLEX WITH A CDRP-RELATED SUBSTRATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-06-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.911 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 81.638, 81.638, 156.745 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.241 * |
| Rwork | 0.241 |
| R-free | 0.31900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.041 * | 0.250 * |
| Number of reflections | 17306 | |
| <I/σ(I)> | 24.1 | 3.5 |
| Completeness [%] | 93.7 * | 78 * |
| Redundancy | 8.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 8 * | Wilmanns, M., (1990) Protein Eng., 3, 173. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | potassium phosphate | 0.05 (M) | pH7.5 |
| 2 | 1 | drop | EDTA | 0.005 (M) | |
| 3 | 1 | drop | 1,4-dithioerythritol | 0.002 (M) | |
| 4 | 1 | drop | 0.02 (%(w/v)) | ||
| 5 | 1 | drop | protein | 10-15 (mg/ml) | |
| 6 | 1 | reservoir | potassium phosphate | 0.05 (M) | pH5.0 |
| 7 | 1 | reservoir | ammonium sulfate | 1.2 (M) | |
| 8 | 1 | reservoir | EDTA | 0.005 (M) | |
| 9 | 1 | reservoir | 1,4-dithioerythritol | 0.002 (M) |
