1IG8
Crystal Structure of Yeast Hexokinase PII with the correct amino acid sequence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1998-09-10 |
Detector | MARRESEARCH |
Wavelength(s) | 1.38 |
Spacegroup name | I 4 |
Unit cell lengths | 142.809, 142.809, 58.462 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 13.000 - 2.200 |
Rwork | 0.162 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | P152K mutant of yeast hexokinase |
RMSD bond length | 0.011 |
RMSD bond angle | 0.033 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.000 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.113 * | 0.420 |
Number of reflections | 60395 | |
Completeness [%] | 95.1 | 96.6 |
Redundancy | 2.12 | 1.82 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.5 | 298 | 2.3-3.2M Ammonium sulphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 1.6-2.4 (M) | |
2 | 1 | reservoir | potassium phosphate | 100 (mM) |