1HU8
CRYSTAL STRUCTURE OF THE MOUSE P53 CORE DNA-BINDING DOMAIN AT 2.7A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2000-06-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0801 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 73.676, 119.992, 184.238 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.700 |
| R-factor | 0.239 * |
| Rwork | 0.239 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tsr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 25.000 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 * | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.051 | 0.189 * |
| Total number of observations | 121009 * | |
| Number of reflections | 22733 | |
| <I/σ(I)> | 9.6 | 3.9 |
| Completeness [%] | 99.5 | 98.7 * |
| Redundancy | 5.3 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 4000, KCl and MgCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 8 (%) | |
| 2 | 1 | reservoir | 200 (mM) | ||
| 3 | 1 | reservoir | 50 (mM) | ||
| 4 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 5 | 1 | reservoir | Tris-HCl | 50 (mM) |
