1F5A
CRYSTAL STRUCTURE OF MAMMALIAN POLY(A) POLYMERASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-10-08 |
Detector | BRANDEIS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.550, 62.720, 179.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.22 * |
Rwork | 0.219 |
R-free | 0.26100 |
RMSD bond length | 0.007 |
RMSD bond angle | 22.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.670 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.085 | 0.267 |
Number of reflections | 19890 | |
<I/σ(I)> | 16.7 | |
Completeness [%] | 87.0 | 65.1 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6 | 24 * | used microseeding * |
1 | Vapor diffusion * | 6 | 24 * | used microseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 22 (%(w/v)) | |
2 | 1 | reservoir | MES | 100 (mM) | |
3 | 1 | reservoir | ammonium sulfate | 120 (mM) | |
4 | 1 | reservoir | 5 (mM) | ||
5 | 1 | reservoir | 2 (mM) | ||
6 | 1 | reservoir | beta-mercaptoethanol | 2 (mM) |