1EI3
CRYSTAL STRUCTURE OF NATIVE CHICKEN FIBRINOGEN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 1999-10-29 |
| Detector | BRANDEIS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 114.726, 101.849, 210.232 |
| Unit cell angles | 90.00, 106.71, 90.00 |
Refinement procedure
| Resolution | ? - 5.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 5.000 | 5.500 * |
| Rmerge | 0.090 | |
| Total number of observations | 45940 * | |
| Number of reflections | 16412 * | |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 80.0 | 65.4 * |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 298 | PEG 3350, imidazole, calcium chloride, pH 6.75, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | 0.15 (M) | ||
| 3 | 1 | drop | imidazole | 0.05 (M) | |
| 4 | 1 | reservoir | PEG3350 | 3-4 (%(v/v)) | |
| 5 | 1 | reservoir | imidazole | 0.05 (M) | |
| 6 | 1 | reservoir | 2 (mM) | ||
| 7 | 1 | reservoir | sodium azide | 1 (mM) |
