1C3C
T. MARITIMA ADENYLOSUCCINATE LYASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-02-19 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 120.809, 126.753, 169.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
Rwork | 0.179 |
R-free | 0.20700 |
RMSD bond length | 0.015 |
RMSD bond angle | 2.640 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.400 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.059 | 0.295 |
Number of reflections | 117163 | |
<I/σ(I)> | 21.67 | 3.1 |
Completeness [%] | 97.8 | 84.1 |
Redundancy | 4 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.5 | 18 * | 100 MM NAACETATE, 0.5% PEG 4000, 6% GLYCEROL, pH 4.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12.5 (mg/ml) | |
2 | 1 | reservoir | sodium acetate | 0.1 (M) | |
3 | 1 | reservoir | PEG4000 | 0.1-0.5 (%) | |
4 | 1 | reservoir | glycerol | 6 (%(v/v)) | |
5 | 1 | reservoir | beta-mercaptoethanol | 1 (mM) | |
6 | 1 | reservoir | 0.02 (%(w/v)) |